This data includes the soil microbial composition data in permafrost of different ages in Barrow area of the Arctic. It can be used to explore the response of soil microorganisms to the thawing in permafrost of different ages. This data is generated by high through-put sequencing using the earth microbiome project primers are 515f – 806r. The region amplified is the V4 hypervariable region, and the sequencing platform is Illumina hiseq PE250; This data is used in the articles published in cryosphere, Permafrost thawing exhibits a greater influence on bacterial richness and community structure than permafrost age in Arctic permafrost soils. The Cryosphere, 2020, 14, 3907–3916, https://doi.org/10.5194/tc-14-3907-2020https://doi.org/10.5194/tc-14-3907-2020 . This data can also be used for the comparative analysis of soil microorganisms across the three poles.
In order to describe the diseases of the main domesticated animals in the Qinghai Tibet Plateau and its surrounding areas, investigate the epidemic situation of the main domestic animals in the Qinghai Tibet Plateau, collect the genetic samples of the resistant and susceptible individuals and the intestinal microbial samples of the main epidemic diseases of the main domestic animals. The data set includes 48 samples of brown cattle in Yili area of Xinjiang, 39 samples of Haidong Mongolian sheep in Qinghai, 32 samples of Qinghai horse, 20 samples of Shangri La yellow cattle in Yunnan and 20 samples of goat. All the samples were fresh feces, and the results of 16S sequencing were obtained after DNA extraction. All the data are original data without any analysis. The purpose of testing these samples is to compare the differences of intestinal microbial species and quantity among different domestic animals in the pan third polar region.
The glacial bacterial resource database of the Tibetan Plateau provides the bacterial 16S ribosomal RNA gene sequences of several glaciers, which are seven glaciers of the Tibetan Plateau separated by an experimental group led by Yongqin Liu during 2010 to 2018 (East Rongbuk Glacier of Mt. Qomolangma, Tianshan Glacier No.1, Guliya Glacier, Laohugou Glacier, Muztagh Ata Glacier, Qiyi Glacier and Yuzhufeng Glacier), the Malan Glacier separated by Shurong Xiang and the Puruogangri Glacier separated by Xinfang Zhang. After the glacier samples were collected, they were taken to the Ecological Laboratory of the Institute of Tibetan Plateau Research of the Chinese Academy of Sciences in Beijing and the National Cryosphere Laboratory in Lanzhou. After applying the spread plate method, the samples were cultured at different temperatures (4-25 °C) for 20 days to 90 days, and single colonies were picked out for purification. After the DNA was extracted from the isolated bacteria, the 16S ribosomal RNA gene fragment was amplified with 27F/1492R primer and sequenced using the Sanger method. The 16S ribosomal RNA gene sequence was compared with the RDP database using the "Classifier" software and identified as level one when the reliability exceeded 80%. These data contain the 16S ribosomal RNA gene fragment sequence and glacier sources of each sequence. Compared with sequences based on high-throughput sequencing, these data have a longer sequence and more accurate classification and can better serve in glacier microbiology research.
The data set of prokaryotic microorganism distribution in the snow and ice of the Arctic Antarctic and the Tibetan Plateau provides the bacterial 16S ribosomal RNA gene sequence collected by the experimental group led by Yongqin Liu from the NCBI database during 2010 to 2018. The keywords for NCBI database search are Antarctic, Arctic Tibetan, and Glacier. The collected sequences were calculated using the DOTOUR software to obtain the similarities between sequences, the sequences with similarities above 97% were clustered into one OTU, and the OTU representative sequence was defined. The OTU representative sequence was compared with the RDP database by the "Classifier" software and was identified as level one when the reliability exceeded 80%. After acquiring the sequence, the GPS coordinates of the sample were obtained by reading the sample information in the sequence file. These data contain the sequence of 16S ribosomal RNA gene fragments for each sequence, evolutionary classification, and sample GPS coordinates. Compared with sequences based on high-throughput sequencing, these data have a longer sequence and more accurate classification. It is significant for comparing the evolutionary information of three-pole microorganisms and understanding the evolution of psychrophilic microorganisms.
The data set of bacterial diversity in Tibetan soil provides the microbial distribution characteristics of the soil surface (0-2 cm) of the Tibetan Plateau. The samples were collected from July 1st to July 15th, 2015, from three types of ecosystems: meadows, grasslands and desert. The soil samples were stored in ice packs and transported to the Ecological Laboratory of the Institute of Tibetan Plateau Research in Beijing. The DNA from the soil was extracted using an MO BIO Power Soil DNA kit. The soil surface samples were stored in liquid nitrogen after collection, shipped to the Sydney laboratory, and then extracted using a Fast Prep DNA kit. The extracted DNA samples adopted 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 909r (5'-GGACTACHVGGGTWTCTAAT-3') to amplify the 16S rRNA gene fragments. The amplified fragments were sequenced by the Illumina Miseq PE250 method, and the raw data were analyzed using Mothur software. The sequences with poor sequencing quality were first removed; the sequences were sorted, and the chimeric sequences were removed. The similarities between the sequences were then calculated, the sequences with similarities above 97% were clustered into one OTU, and the OTU representative sequence was defined. The OTU representative sequence was compared with the Silva database and identified as level one when the reliability exceeded 80%. The microbial diversities in these data on the Tibetan Plateau were systematically compared, which made them significant to the study of the microbial distribution on the Tibetan Plateau.
The Antarctic and Arctic bacterial distribution data set provides distribution characteristics of bacteria in the Arctic and Antarctic. The collection period of the samples was from December 13,2005, to December 8,2006; 52 samples were obtained from 3 Arctic regions (Spitsbergen Slijeringa, Spitsbergen Vestpynten, and Alexandra Fjord_Highlands), and 171 samples were obtained from 5 Antarctic regions (the Mitchell Peninsula, Casey station main Power house, Robinsons Ridge, Herring Island, and Browning Peninsula). The soil surface samples were stored in liquid nitrogen after collection, shipped to a Sydney laboratory, and extracted using the FastPrep DNA kit. The extracted DNA samples were processed by 27F (5'-GAGTTTGATCNTGGCTCA-3' and 519R (5'-GTNTTACNGCGGCKGCTG-3') to amplify the 16S rRNA gene fragments. The amplified fragments were sequenced by the 454 method, and the raw data were analyzed by Mothur software. First, the sequences with poor sequencing quality were removed, the sequences were then sorted, and the chimera sequences were removed. The similarities between the sequences were calculated, the sequences with similarities above 97% were clustered into one OTU, and the OTU representative sequence was defined. By comparison with the Silva database, the OTU sequences with reliabilities greater than 80% were identified as level one. This data system compared the diversity of microorganisms in the eastern Antarctic with that in the Arctic and is of great significance for the study of the distributions of microorganisms in the Antarctic and Arctic.
Microbial diversity data of lakes on the Tibetan Plateau. One hundred and thirty-eight samples were collected from July 1st to July 15th, 2015, from 28 lakes (Bamco, Baima Lake, Bange Salt Lake, Bangong Lake, Bengco, Bieruozeco, Cuoeco, Cuoe (Pingcuo North), Dawaco, Dangqiongco, Dangreyongco, Dongco, Eyacuoqiong, Gongzhuco, Guogenco, Jiarebuco, Mapangyongco, Namco, Nieerco (Salt Lake), Normaco, Pengyanco, Pengco, Qiangyong, Selinco, Wuruco, Wumaco, Zharinanmuco, and Zhaxico). The salinity gradients range from 0.07-118 ppm. The DNA extraction method: The DNA was extracted using an MO BIO PowerSoil DNA kit after the lake water was filtered onto a 0.45 membrane. The 16S rRNA gene fragment amplification primers were 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 909r (5'-GGACTACHVGGGTWTCTAAT-3'). The sequencing method was Illumina MiSeq PE250, and the raw data were analyzed by Mothur software, including quality filtering and chimera removal. The sequence classification was based on the Silva109 database, and archaea, eukaryotic and unknown source sequences have been removed. OTUs were classified by 97% similarity, and sequences that appear once in the database were then removed. Finally, each sample was resampled to 7,230 sequences/sample. GPS coordinates, evolutionary information, and environmental factors are listed in the data.