The data set include crop leaf stomatal conductance observed at four sample regions, that is the soil moisture control experimental field at Daman county, and the super station, and Shiqiao sample plots at Wuxing village in Zhangye city. 1) Objective Crop leaf stomatal conductance, a key biophysical parameter, was observed as model parameter or a priori knowledge for crop growth model, or evapotranspiration estimation. 2) Measuring instruments Leaf porometer. 3) Measuring site a. the soil moisture control experimental field at Daman county, Twelve soil water treatments are set. The crop leaf stomatal conductance for each treatment is measured on 17, 23 and 29 May, and 3, 9, 14 and 24 June, and 5 and 12 July. b. the Super Station The crop leaf stomatal conductance at the super station is measured on 22 and 28 May, 5, 11, 18, and 25 June, and 1, 8, 15, 22 and 31 July, 9, 15 and 22 August, and 3 and 11 September. c. the Shiqiao sample site The crop leaf stomatal conductance at the Shiqiao village is measured on 17, 22 and 28 May, 4, 11, 17 and 25 June, 1, 8, 15, 22, and 30 July, 8, 16 and 27 August, and 9 September. 4) Data processing The observational data was recorded in the sheets and reorganized in the EXCEL sheets. The time used in this dataset is in UTC+8 Time.
Xu Fengying Wang Jing Zhuang Jinxin Huang Yongsheng LI Xin MA Mingguo
The dataset combined with crop phrenology data and field management data which were investigated near the 13 eddy covariance (EC) stations. 1.1 Objective of investigation Objectives of investigation is to supply assistant information for experiment on EC, meteorology, and biophysics parameter. 1.2 Investigation spots and items Investigation spots include Jiu She of Shiqiao village (EC3), Xiaoman southern road (EC16), Wu She of Five stars village (EC13), Wu She of Xiaoman village (EC14), Er She of Shiqiao village (EC5), Liu She of Zhonghua village (EC11), Liu She of Shiqiao village (EC2), Wu She of JinCheng village (EC7), EC6, Liu She of Jincheng village (EC8), Yi She of Kangning village (EC9), Er She of Kangning village (EC10), and Si She of Jingcheng village (EC12). Investigation items comprise crop type, crop name, seed time, seed type, plant span, row span, field area, germination time, three leaves period, seven leaves period, farming way, farming time, irrigation time, irrigation water volume, fertilization time, fertilization type, and fertilization rate. The time used in this dataset is in UTC+8 Time. 1.3 Data collection Data was collected by using ask-reply approach according to investigation tables.
Gai Yingchun Zhuang Jinxin Ma Chunfeng Zhuang Jinxin LI Xin
一. Data overview This data interchange is the second data interchange of "genomics research on drought tolerance mechanism of typical desert plants in heihe basin", a key project of the major research program of "integrated research on eco-hydrological processes in heihe basin".The main research goal of this project is a typical desert sand Holly plants as materials, using the current international advanced a new generation of gene sequencing technology to the whole genome sequence and gene transcription of Holly group sequence decoding, so as to explore related to drought resistance gene and gene groups, and transgenic technology in model plants such as arabidopsis and rice) verify its drought resistance. 二, data content 1.Sequencing of the genome and transcriptome of lycophylla SPP. The genome size of Mongolian Holly was about 926 Mb, GC content 36.88%, repeat sequence proportion 66%, genome heterozygosity rate 0.56%, which indicated that the genome has many repeat sequences, high heterozygosity and belongs to a complex genome.Based on the predicted sequence results, we subsequently carried out in-depth sequencing of the genome of lysiopsis SPP. The obtained data were assembled to obtain a 937 Mb genome sequence (table 1), which was basically the same as the predicted genome size.Through to the sand Holly transcriptome sequencing and sequence assembly (table 2), received more than 77000 genes coding sequence (Unigene), these sequences are comments found that most of the gene sequence and legumes and soybean, garbanzo beans and bean has a higher similarity (figure 1), consistent with the fact of sand ilex leguminous plants. 一), and the sand Holly is a leguminous plants consistent with the fact. 2.Discovery of simple repeat sequence (SSR) molecular markers of sand Holly: There is a transcriptome data set of sand Holly in the network public database, and the sample collection site is zhongwei city, ningxia.But this is the location of the project team samples in minqin county, gansu province, in order to study whether this sand in different areas of the Holly sequence has sequence polymorphism, we first identify the minqin county plant samples in the genomes of simple sequence repeat (SSR) markers (table 3), and then, compares the transcriptome sequences of plant sample, found in part of SSR molecular marker polymorphism (table 4), these molecular markers could be used for the species of plant genetic map construction, QTL mapping and genetic diversity analysis in the study. 三, data processing instructions Sample collection place: minqin county, gansu province, latitude and longitude: N38 ° 34 '25.93 "E103 ° 08' 36.77".Genome sequencing: a total of 8 genomic DNA libraries of different sizes were constructed and determined by Illumina HiSeq 2500 instrument.Transcriptome sequencing: a library of 24 transcriptome mrnas was constructed and determined by Illumina HiSeq 4000. 四, the use of data and meaning We selected a typical desert plant as the research object, from the Angle of genomics, parse the desert plant genome and transcriptome sequences, excavated its precious drought-resistant gene resources, and to study their drought resistance mechanism of favorable sand Holly this ancient and important to the utilization of plant resources, as well as the heihe river basin of drought-resistant plant genetic breeding, ecological restoration and sustainable development.
HE Junxian FENG Lei
In the previous project, three different types of desert investigation and observation sites in the lower reaches of Heihe River were set up. Different kinds of desert plants with the same average growth and size as the observation site were selected for the above ground biomass and underground biomass total root survey. The dry weight was the dry weight at 80 ℃, and the root shoot ratio was the dry weight ratio of the underground biomass to the aboveground biomass. Species: Elaeagnus angustifolia, red sand, black fruit wolfberry, bubble thorn, bitter beans, Peganum, Tamarix and so on.
"Hydrological ecological economic process coupling and evolution of Heihe River Basin Management under the framework of water rights" (91125018) project data collection 2 - Dunhuang comprehensive plan for rational utilization of water resources and ecological protection (2011-2020) Planning documents mainly include: 1. Current situation and existing problems of regional water resources utilization; 2. Guiding ideology, basic principles and planning objectives; 3. Analysis of economic, social and ecological water demand; 4. Plan for water resources allocation; 5. Construction of water right system; 6. Main engineering measures; 7. Environmental impact arrangement.
"Coupling and Evolution of Hydrologic -Ecologic-Economic Processes of the Heihe River Basin Under the Framework of Water Rights" (91125018) Project data collection 1 - SWater Resources Improvement Plan of Shiyang River Basin 1. Data Overview:The improvement plan of Shiyang River Basin was implemented in 2007 for river basin comparison. 2. Data Content: The released plan.
The data set is the physiological and ecological parameters of the dominant species of each ecosystem in Heihe River Basin. According to the requirements of tesim model, the data set divides Heihe River basin into seven ecosystems: deciduous broad-leaved forest ecosystem (BRD), evergreen coniferous forest ecosystem (CNF), agricultural field ecosystem (CRP), desert ecosystem (DST), meadow grassland ecosystem (MDS) Shrubbery ecosystem (SHB) and grassland ecosystem (STP). Some of the data in this data set are based on the measured data, some are obtained by reference documents, but after verification, they are applied to the Heihe River Basin. For the data in this data, each parameter of each ecosystem has three values, which are the value in the model, the minimum value and the maximum value of this parameter. The data can provide input parameters for the ecological process model, and the data set is still in further optimization.
This data set is collected according to the output results of tesim ecological process model, including biomass, plant N and P content, evapotranspiration, NPP and other model output results. Some of the results are obtained by field measurement, some by laboratory analysis of field samples, some by literature.
Background: this data interchange is the first data interchange of the key project of "integrated study of eco-hydrological processes in heihe basin", "genomics research on drought tolerance mechanism of typical desert plants in heihe basin".The main research targets of the key projects is a typical sand desert plants are Holly, using the current international advanced a new generation of gene sequencing technology to the whole genome sequence and gene transcription of Holly group sequence decoding, so as to explore related to drought resistance gene and gene groups, and transgenic technology in model to verify their drought resistance in plants. Process and content: as genome sequencing requires special sequencing equipment, the project is huge and the process is complex (mainly including genome library construction, sequencing, data analysis and genome assembly), so it needs to be completed by a professional sequencing company.After contacting with sequencing companies, we learned that before sequencing an unknown genome, the size and complexity of the genome should be predicted, which is a necessary prerequisite for designing sequencing schemes and strategies.Therefore, in 2013, we mainly predicted the chromosome composition, genome size and complexity of sand Holly, and successfully established the extraction and purification method of its genomic DNA.The results showed that the plant was diploid, the genome was composed of 9 staining lines (18 lines of diploid), and the genome size was 1.07G.The quality test results of the genomic DNA indicated that the requirements of the obtained DNA complex sequencing have been sent to the sequencing company for library construction and sequencing, which is now in progress.In addition, in order to obtain a large number of uniform plant materials, we have discussed the induction of callus, which has been successful.Due to these reasons, we were unable to complete the genome sequencing and submit the relevant data of sand Holly in accordance with the original plan of the project this year, mainly because we did not count the predicted contents of the genome before. Data usage: the data obtained in this year on ploidy, karyotype composition and genome size of lycopodium SPP.The success of the callus induction provides a high-quality material guarantee for the subsequent transcriptome sequencing and drought-resistance mechanism research experiments, and it is also a new contribution to the cytological and physiological research of the plant.
HE Junxian GU Lifei
The dataset investigated the growth status of plants and leaf morphological indexes of single and conjoined red sand and pearl in the middle and lower reaches of heihe river basin in 2013. The growth indexes were crown width, plant height, and biomass of fine roots and thick roots.Leaf shape indicators are: length, width, thickness, and leaf area, volume, etc.The experimental observation indexes are: leaf nitrogen content, water potential, gas exchange data, chlorophyll fluorescence data. Data include: field observation data and explanatory documents.
As determined in mid-august 2013, planting species: bubbly spines (different habitats are mid-range intermountain lowland and gobi), red sand (different habitats are mid-range gobi and downstream gobi). Using the brother company of LI - 6400 Portable Photosynthesis System (Portable Photosynthesis System, LI - COR, USA) and LI - 3100 leaf area meter, etc., to the desert plant photosynthetic physiological characteristics were observed. The symbolic meaning of the observed data is as follows: Obs，observation frequency ； Photo ，net photosynthetic rate，μmol CO2•m–2•s–1； Cond stomatal conductance，mol H2O•m–2•s–1 ; Ci, Intercellular CO2 concentration, μmol CO2•mol-1； Trmmol，transpiration rate，mmol H2O•m–2•s–1； Vpdl，Vapor pressure deficit，kPa； Area，leaf area，cm2； Tair，free air temperature ，℃； Tleaf，Leaf temperature，℃； CO2R，Reference chamber CO2 concentration，μmol CO2•mol-1； CO2S，Sample chamber CO2 concentration，μmol CO2•mol-1； H2OR，Reference chamber moisture，mmol H2O•mol-1； H2OS，Sample chamber moisture，mmol H2O•mol-1； PARo，photon flux density，μmol•m–2•s–1； RH-R，Reference room air relative humidity，%； RH-S，Relative humidity of air in sample room，%； PARi，Photosynthetic effective radiation，μmol•m–2•s–1； Press，barometric pressure，kPa； Others are the state parameters of the instrument at the time of measurement.
On the basis of physiological and biochemical analysis of photosynthetic organs (leaves or assimilating branches) of typical desert plants in heihe river basin collected in mid-july 2011, some photosynthetic organs of desert plants were collected in mid-july 2012 and put into a liquid nitrogen tank and brought back to the laboratory for determination. Physiological analysis indexes mainly include: soluble protein unit: mg/g;Free amino acid unit: g/g;Chlorophyll content unit: mg/g;Superoxide dismutase (SOD) unit: U/g FW;Catalase (CAT) unit: U/(g•min);POD unit: U/(g•min);Proline (Pro) unit: g/g; Soluble sugar unit: g/g;Malondialdehyde (MDA) is given in moles per liter.
At the end of September and the beginning of October, 2013, desert plants in typical areas of heihe basin stopped their growth period to conduct year-end ecological survey. There are altogether 8 survey and observation fields, which are: piedmont desert, piedmont gobi, middle reaches desert, middle reaches gobi, middle reaches desert, lower reaches desert, lower reaches gobi and lower reaches desert, with a size of 40m×40m. Three 20m×20m large quadrats were fixed in each observation field, named S1, S2 and S3, and regular shrub surveys were conducted.Each large quadrat was fixed with 4 5m x 5m small quadrats, named A, B, C, D, for the herbal survey.
In mid-july 2011, photosynthetic organs (leaves or assimilating branches) of typical desert plants were collected and brought back to the laboratory in a liquid nitrogen tank for determination. The analysis indexes mainly include soluble protein unit: mg/g;Free amino acid unit: g/g;Chlorophyll content unit: mg/g;Superoxide dismutase (SOD) unit: U/g FW;Catalase (CAT) unit: U/(g•min);POD unit: U/(g•min);Proline (Pro) unit: g/g; Soluble sugar unit: g/g;Malondialdehyde (MDA) is given in moles per liter.
The annual total net primary productivity (NPP) and average productivity of different ecosystems in heihe river basin from 1998 to 2002 were estimated by using the light energy utilization model c-fix, high spatial and temporal resolution remote sensing data of SPOT/VEGETATION, global grid meteorological reanalysis data and land use map of heihe river basin. From 1998 to 2002, the 10-day 1-km resolution SPOT VEGETATATION NDVI (10-day maximum synthesis) data product in the heihe basin, provided by the image processing and archiving center (CTIV) of VITO institute, Belgium, was used to calculate the key parameters fAPAR required by the c-fix model. The daily temperature and total radiation of heihe river basin from 1998 to 2002 were obtained using a global 1.5 °× 1.5 ° grid meteorological data product from MeteoFrance. It contains the spatial distribution pattern of annual accumulation of NPP in heihe basin and the seasonal dynamic map of NPP.The spatial resolution of this data is 1km.
The data set mainly includes observation data of each tree in the super site, and the observation time is from June 2, 2008 to June 10, 2008. The super site is set around the Dayekou Guantan Forest Station. Since the size of the super site is 100m×100m, in order to facilitate the forest structure parameter survey, the super site is divided into 16 sub-sample sites, and tally forest measurement is performed in units of sub-samples. The tally forest measurement factors include: diameter, tree height, height under branch, crown width in transversal slope direction, crown width in up and down slope direction, and tindividual tree growth status. The measuring instruments are mainly: tape, diameter scale, laser altimeter, ultrasonic altimeter, range pole and compass. The data set also records the center point latitude and longitude coordinates of 16 sub-samples (measured by Z-MAX DGPS). The data set can be used for verification of remote sensing forest structure parameter extraction algorithm. The data set, together with other observation data of the super site, can be used for reconstruction of forest 3D scenes, establishment of active and passive remote sensing mechanism models, and simulation of remote sensing images,etc.
CHEN Erxue Lina Bai Bengyu Wang Xin Tian LIU Qingwang CAO Bin Yang Yongtian Zhihai Gao Bingxiang Tan GUO Zhifeng WANG Xinyun FU Anmin ZHANG Zhiyu NI Wenjian WANG Qiang BAO Yunfei WANG Dianzhong ZHANG Yang ZHAO Liqiong LIANG Dashuang WANG Shunli ZHAO Ming LEI Jun NIU Yun LUO Longfa
The main contents of this data set are forest, shrub and grassland sample plot survey data.The fixed samples are located in the drainage ditch valley of qilian mountain and the dayaokou valley where the hydrology observation and test site of the water source conservation forest research institute of gansu province is located. The information of the sample is as follows: Number elevation quadrat size longitude latitude surface type G1 2715 20 × 20 100 ° 17 '12 "38 ° 33' 29" qinghai spruce forest G2 2800 20×36 100°17 '07 "38°33' 27" moss spruce forest G3 2840 20×20 100°17 '37 "38°33' 05" moss spruce forest G4 2952 20 × 20 100 ° 17 '59 "38 ° 32' 47" qinghai spruce forest G5 3015 20 × 20 100 ° 18 '06 "38 ° 32' 42" qinghai spruce forest G6 3100 20 × 20 100 ° 18 '13 "38 ° 32' 31" thicket qinghai spruce forest G7 3300 23.5 × 20 thickets qinghai spruce forest G8 2800 20×20 100°13 '30 "38°33' 29" moss spruce forest B1 2700 12.8×25 moss spruce forest B2 2800 20×20 100°17 '38 "38°32' 59" moss spruce forest B3 2900 20×20 100°17 '59 "38°32' 51" grass spruce forest B4 3028 20×20 100°17 '59 "38°32' 39" moss spruce forest B5 3097 20×20 100°18 '02 "38°32' 32" moss spruce forest B6 3195 20 × 20 100 ° 18 '06 "38 ° 32' 25" qinghai spruce forest B7 2762 20 × 20 100 ° 17 '08 "38 ° 33' 21" qinghai spruce forest B8 2730 20×20 100°17 '06 "38°33' 27" moss spruce forest GM1 3690 5×5 100°18 '02 "38°32' 02" caragana scrub (middle) GM2 3690 5×5 100°18 '02 "38°32' 02" caragana scrub (rare) GM3 3700 5×5 100°18 '03 "38°32' 03" caragana + jilaliu shrub (dense) GM4 3600 5×5 100°18 '10 "38°32' 06" caragana + jila willow thicket (middle) GM5 3600 5×5 100°18 '10 "38°32' 06" caragana + jila willow shrub (sparse) GM6 3600 5×5 100°18 '10 "38°32' 06" caragana + jila willow thicket (dense) GM7 3500 5×5 100°18 '14 "38°32' 08" caragana + jila willow thicket (middle) GM8 3500 5×5 100°18 '14 "38°32' 08" caragana + jila willow thicket (dense) GM9 3500 5×5 100°18 '14 "38°32' 08" caragana + jila willow thicket (rare) GM10 3400 5×5 100°18 '18 "38°32' 12" golden pheasant scrub (rare) GM11 3400 5×5 100°18 '18 "38°32' 12" golden pheasant + golden raspberry shrub (dense) GM12 3400 5×5 100°18 '18 "38°32' 12" golden pheasant scrub (rare) GM13 3300 5 × 5 100 ° 18 '21 "38 ° 32' 21" giraliu thicket GM14 3300 5 × 5 100 ° 18 '21 "38 ° 32' 21" caragana + jila shrub GM15 3300 5 × 5 100 ° 18 '21 "38 ° 32' 21" caragana + jila shrub YC3 2700 1×1 100°17 '14 "38°33' 33" needle thatch field YC4 2750 1×1 100°17 '18 "38°33' 32" needle thatch field YC5 2800 1×1 100°17 '21 "38°33' 33" needle thatch field YC6 2850 1×1 100°17 '25 "38°33' 33" needle thatch field YC7 2900 1×1 100°17 '31 "38°33' 32" aster + needle thatch field YC8 2950 1×1 100°17 '44 "38°33' 23" needle thatch field YC9 2980 1×1 100°17 '48 "38°33' 25" needle thatch field The sample geodesic tree data were surveyed from July to August 2007.The survey included: 1. Basic survey of sample plots in drainage ditch basin: A) sample land setting: sample land number, elevation, slope direction, slope position, slope, soil layer thickness, sample land size, longitude and latitude, community type, soil type, operation status, age B) survey of each wood in the sample plots: sample plot number, tree number, tree species, tree classification, chest diameter, tree height, undershoot height, crown radius 2. Soil profile survey record sheet Including forest/vegetation status, major tree species, forest age, soil name, surface soil erosion, parent rock and material, drainage conditions, land use history, soil profile (soil layer, moisture, color, texture, structure, root system, gravel content) 3. Standard ground cover factor Standard land area, dominant tree species, stand/vegetation origin, elevation, slope direction, slope position, slope, cutting and utilization method, afforestation land preparation type, survey method, canopy coverage, living ground cover, dead cover cover, litter thickness (undivided strata, semi-decomposed layer, decomposed layer) 4. Canopy survey: 5. Draft quadrat (1m×1m) survey record sheet Including species name, number, coverage, average height 6. Results of determination of soil physical properties in source forest of qilian mountain (land sample survey) Contains the soil physical properties measurement process (+ wet mud weight aluminum box, aluminum box, soil moisture content, suddenly bulk density, etc.), bringing biomass measurement (total fresh weight of shrub and herb, fresh weight of sample, sample dry weight, etc.), litter dry weight (including mosses) layer and the largest capacity calculation process (of moss and litter thickness, total fresh weight, fresh weight of samples, the dry weight of the sample, soaking for 24 h after heavy, maximum water holding capacity, the largest water depth, the biggest hold water rate, maximum moisture capacity) 7. Bush sample survey: Including species name, number, coverage, average height 8. Standard sample land setting and questionnaire for each wooden inspection ruler Including tree species, tree classification, age, chest diameter, number of height, undershoot height, crown radius 9. Litter layer survey record sheet Including litter (decomposed layer, semi-decomposed layer, decomposed layer) thickness 10. Update survey records: Including tree species, natural regeneration (height <30cm, height 31-50cm, height >51cm), artificial regeneration (height <30cm, height 31-50cm, height >51cm) This data set can provide ground measured data for remote sensing inversion of forest structure parameters.
WANG Shunli LUO Longfa WANG Rongxin CHE Zongxi JING Wenmao
The forest hydrology experimental area of Heihe River integrated remote sensing experiment includes the dense observation area of Dayekou basin and the dense observation area of Pailugou basin. Due to the concentrated distribution of the fixed sample plots in the drainage ditch basin, these sample plots lack of representativeness to the forest of the whole dayokou basin, so in June 2008, 43 temporary forest sample plots were set up in the whole dayokou basin. The data set is the ground observation data of the 43 temporary plots. In addition to the measurement and recording of stand status and site factors, Lai was also observed. The instruments used to measure each wood in the sample plot are mainly tape, DBH, flower pole, tree measuring instrument and compass. The DBH, tree height, height under branch, crown width in cross slope direction, crown width along slope direction and single tree growth were measured for each tree. WGS84 latitude and longitude coordinates of the center point of the sample plot were measured with different hand-held GPS, and the positioning error was about 5-30m. Other observation factors include: Forest Farm, slope direction, slope position, slope, soil thickness, canopy density, etc. The implementation time of these temporary sample plots is from 2 to 30 June 2008. The data set can provide ground data for the development of remote sensing inversion algorithm of forest structure parameters.
LING Feilong HE Qisheng ZHANG Xuelong WANG Shunli ZHAO Ming LEI Jun NIU Yun LUO Longfa CHEN Erxue